TESNinSAB project mostly relies on toxicity experiments with E. crypticus and Ag measurement in soil, porewater and animals. For lethal and reproductitive toxcicity, standard methods are used. Some steps that I have applied in my laboratory are as follows:
Preparing Ag nanoparticle solutions:
Spiking Ag nanoparticles to soil:
Taking sample for pH measurement:
Samples for pH measurement:
Introducing animals to soil:
Toxicty samples are ready to wait for 3 weeks:
Ending the toxicty experiment:
Counting the animals:
